Single molecule mechanics of the kinesin neck.

@article{Bornschlgl2009SingleMM,
  title={Single molecule mechanics of the kinesin neck.},
  author={Thomas Bornschl{\"o}gl and Guenther Woehlke and Matthias Rief},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2009},
  volume={106 17},
  pages={6992-7}
}
Structural integrity as well as mechanical stability of the parts of a molecular motor are crucial for its function. In this study, we used high-resolution force spectroscopy by atomic force microscopy to investigate the force-dependent opening kinetics of the neck coiled coil of Kinesin-1 from Drosophila melanogaster. We find that even though the overall thermodynamic stability of the neck is low, the average opening force of the coiled coil is >11 pN when stretched with pulling velocities… CONTINUE READING

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