Single-molecule measurement of protein folding kinetics.

@article{Lipman2003SinglemoleculeMO,
  title={Single-molecule measurement of protein folding kinetics.},
  author={Everett A Lipman and Benjamin M Schuler and Olgica Bakajin and William Allen Eaton},
  journal={Science},
  year={2003},
  volume={301 5637},
  pages={1233-5}
}
In order to investigate the behavior of single molecules under conditions far from equilibrium, we have coupled a microfabricated laminar-flow mixer to a confocal optical system. This combination enables time-resolved measurement of Förster resonance energy transfer after an abrupt change in solution conditions. Observations of a small protein show the evolution of the intramolecular distance distribution as folding progresses. This technique can expose subpopulations, such as unfolded protein… CONTINUE READING

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