Single-molecule imaging of receptor-receptor interactions.

@article{Suzuki2013SinglemoleculeIO,
  title={Single-molecule imaging of receptor-receptor interactions.},
  author={Kenichi G. N. Suzuki and Rinshi S. Kasai and Takahiro K Fujiwara and Akihiro Kusumi},
  journal={Methods in cell biology},
  year={2013},
  volume={117},
  pages={373-90}
}
Single-molecule imaging is a powerful tool for the study of dynamic molecular interactions in living cell plasma membranes. Herein, we describe a single-molecule imaging microscopy technique that can be used to measure lifetimes and densities of receptor dimers and oligomers. This method can be performed using a total internal reflection fluorescent microscope equipped with one or two high-sensitivity cameras. For dual-color observation, two images obtained synchronously in different colors are… CONTINUE READING