Single-molecule imaging of EGFR signalling on the surface of living cells

@article{Sako2000SinglemoleculeIO,
  title={Single-molecule imaging of EGFR signalling on the surface of living cells},
  author={Yasushi Sako and Shigeru Minoghchi and Toshio Yanagida},
  journal={Nature Cell Biology},
  year={2000},
  volume={2},
  pages={168-172}
}
The early events in signal transduction from the epidermal growth factor (EGF) receptor (EGFR) are dimerization and autophosphorylation of the receptor, induced by binding of EGF. Here we observe these events in living cells by visualizing single molecules of fluorescent-dye-labelled EGF in the plasma membrane of A431 carcinoma cells. Single-molecule tracking reveals that the predominant mechanism of dimerization involves the formation of a cell-surface complex of one EGF molecule and an EGFR… Expand
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