Single-molecule fluorescence resonance energy transfer in molecular biology.

@article{Sasmal2016SinglemoleculeFR,
  title={Single-molecule fluorescence resonance energy transfer in molecular biology.},
  author={Dibyendu Kumar Sasmal and Laura Pulido and Shan Kasal and Jun Huang},
  journal={Nanoscale},
  year={2016},
  volume={8 48},
  pages={
          19928-19944
        }
}
Single-molecule fluorescence resonance energy transfer (smFRET) is a powerful technique for studying the conformation dynamics and interactions of individual biomolecules. In this review, we describe the concept and principle of smFRET, illustrate general instrumentation and microscopy settings for experiments, and discuss the methods and algorithms for data analysis. Subsequently, we review applications of smFRET in protein conformational changes, ion channel open-close properties, receptor… CONTINUE READING