Single-molecule fluorescence measurements of ribosomal translocation dynamics.

  title={Single-molecule fluorescence measurements of ribosomal translocation dynamics.},
  author={Chunlai Chen and Benjamin D. Stevens and Jaskarin Kaur and Diana M Cabral and Hanqing Liu and Yuhong Wang and Haibo Zhang and Gabriel Rosenblum and Zeev Smilansky and Yale E. Goldman and Barry S. Cooperman},
  journal={Molecular cell},
  volume={42 3},
We employ single-molecule fluorescence resonance energy transfer (smFRET) to study structural dynamics over the first two elongation cycles of protein synthesis, using ribosomes containing either Cy3-labeled ribosomal protein L11 and A- or P-site Cy5-labeled tRNA or Cy3- and Cy5-labeled tRNAs. Pretranslocation (PRE) complexes demonstrate fluctuations between classical and hybrid forms, with concerted motions of tRNAs away from L11 and from each other when classical complex converts to hybrid… CONTINUE READING

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