Single-molecule dual-beam optical trap analysis of protein structure and function.

  title={Single-molecule dual-beam optical trap analysis of protein structure and function.},
  author={Jongmin Sung and Sivaraj Sivaramakrishnan and Alexander R Dunn and James A Spudich},
  journal={Methods in enzymology},
Optical trapping is one of the most powerful single-molecule techniques. We provide a practical guide to set up and use an optical trap, applied to the molecular motor myosin as an example. We focus primarily on studies of myosin function using a dual-beam optical trap, a protocol to build such a trap, and the experimental and data analysis protocols to utilize it. 
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