Single-molecule denaturation mapping of DNA in nanofluidic channels.

  title={Single-molecule denaturation mapping of DNA in nanofluidic channels.},
  author={Walter W. Reisner and Niels B. Larsen and Asli Silahtaroglu and Anders Kristensen and Niels Tommerup and Jonas O. Tegenfeldt and Henrik Flyvbjerg},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={107 30},
Here we explore the potential power of denaturation mapping as a single-molecule technique. By partially denaturing YOYO-1-labeled DNA in nanofluidic channels with a combination of formamide and local heating, we obtain a sequence-dependent "barcode" corresponding to a series of local dips and peaks in the intensity trace along the extended molecule. We demonstrate that this structure arises from the physics of local denaturation: statistical mechanical calculations of sequence-dependent… CONTINUE READING