Single molecule blinking and photobleaching separated by wide-field fluorescence microscopy.

@article{Gensch2005SingleMB,
  title={Single molecule blinking and photobleaching separated by wide-field fluorescence microscopy.},
  author={Thomas Gensch and Martin B{\"o}hmer and Pedro Francisco Aramend{\'i}a},
  journal={The journal of physical chemistry. A},
  year={2005},
  volume={109 30},
  pages={6652-8}
}
Single molecule fluorescence detection of Atto590 in poly(vinyl alcohol) was achieved by using a wide-field epifluorescence microscope with CCD-camera detection. Image sequences are obtained from which the time traces of the detected molecules are built. We find a distinctive difference between the time evolution of the fluorescence originating from the… CONTINUE READING