Single molecule analysis reveals reversible and irreversible steps during spliceosome activation

@inproceedings{Hoskins2016SingleMA,
  title={Single molecule analysis reveals reversible and irreversible steps during spliceosome activation},
  author={Aaron A Hoskins and Margaret L. Rodgers and Larry J Friedman and Jeff Gelles and Melissa J. Moore},
  booktitle={eLife},
  year={2016}
}
The spliceosome is a complex machine composed of small nuclear ribonucleoproteins (snRNPs) and accessory proteins that excises introns from pre-mRNAs. After assembly the spliceosome is activated for catalysis by rearrangement of subunits to form an active site. How this rearrangement is coordinated is not well-understood. During activation, U4 must be released to allow U6 conformational change, while Prp19 complex (NTC) recruitment is essential for stabilizing the active site. We used multi… CONTINUE READING