Single-molecule analysis of SSB dynamics on single-stranded DNA.

  title={Single-molecule analysis of SSB dynamics on single-stranded DNA.},
  author={Ruobo Zhou and Taekjip Ha},
  journal={Methods in molecular biology},
SSB proteins bind to and control the accessibility of single-stranded (ss) DNA generated as a transient intermediate during a variety of cellular processes. For subsequent DNA processing, however, SSB needs to be removed and yield to other proteins while avoiding ssDNA exposure to nucleases. Using single-molecule two- and three-color fluorescence resonance energy transfer (FRET) and fluorescence-force spectroscopy, we recently showed that the SSB/DNA complex is a highly dynamic system and SSB… CONTINUE READING

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