Single-molecule PCR: an artifact-free PCR approach for the analysis of somatic mutations.

@article{Kraytsberg2005SinglemoleculePA,
  title={Single-molecule PCR: an artifact-free PCR approach for the analysis of somatic mutations.},
  author={Yevgenya Kraytsberg and Konstantin Khrapko},
  journal={Expert review of molecular diagnostics},
  year={2005},
  volume={5 5},
  pages={809-15}
}
A critical review of the clone-by-clone approach to the analysis of complex spectra of somatic mutations is presented. The study of a priori unknown somatic mutations requires painstaking analysis of complex mixtures of multiple mutant and non-mutant DNA molecules. If mutant fractions are sufficiently high, these mixtures can be dissected by the cloning of individual DNA molecules and scanning of the individual clones for mutations (e.g., by sequencing). Currently, the majority of such cloning… CONTINUE READING

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