Single-molecule FRET unveils induced-fit mechanism for substrate selectivity in flap endonuclease 1

@inproceedings{Rashid2017SinglemoleculeFU,
  title={Single-molecule FRET unveils induced-fit mechanism for substrate selectivity in flap endonuclease 1},
  author={Fahad Rashid and P. D. Harris and Manal S Zaher and Mohamed A. Sobhy and Luay I Joudeh and Chunli Yan and Hubert Piwonski and Susan E. Tsutakawa and Ivaylo Ivanov and John A. Tainer and Satoshi Habuchi and Samir M Hamdan},
  booktitle={eLife},
  year={2017}
}
Human flap endonuclease 1 (FEN1) and related structure-specific 5'nucleases precisely identify and incise aberrant DNA structures during replication, repair and recombination to avoid genomic instability. Yet, it is unclear how the 5'nuclease mechanisms of DNA distortion and protein ordering robustly mediate efficient and accurate substrate recognition and catalytic selectivity. Here, single-molecule sub-millisecond and millisecond analyses of FEN1 reveal a protein-DNA induced-fit mechanism… CONTINUE READING
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