Single-molecule DNA-mapping and whole-genome sequencing of individual cells.

@article{Marie2018SinglemoleculeDA,
  title={Single-molecule DNA-mapping and whole-genome sequencing of individual cells.},
  author={Rodolphe Marie and Jonas N Pedersen and Loic B{\ae}rlocher and Kamila Koprowska and Marie P{\o}denphant and C{\'e}line Sabatel and Maksim Zalkovskij and Andrej Mironov and Brian Bilenberg and Neil Ashley and Henrik Flyvbjerg and Walter F. Bodmer and Anders Kristensen and Kalim U. Mir},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2018},
  volume={115 44},
  pages={
          11192-11197
        }
}
To elucidate cellular diversity and clonal evolution in tissues and tumors, one must resolve genomic heterogeneity in single cells. To this end, we have developed low-cost, mass-producible micro-/nanofluidic chips for DNA extraction from individual cells. These chips have modules that collect genomic DNA for sequencing or map genomic structure directly, on-chip, with denaturation-renaturation (D-R) optical mapping [Marie R, et al. (2013) Proc Natl Acad Sci USA 110:4893-4898]. Processing of… CONTINUE READING

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