Single mimivirus particles intercepted and imaged with an X-ray laser

  title={Single mimivirus particles intercepted and imaged with an X-ray laser},
  author={M. Marvin Seibert and Tomas Ekeberg and Filipe R.N.C. Maia and Martin Svenda and Jakob Andreasson and Olof J{\"o}nsson and Du{\vs}ko Odi{\'c} and Bianca Iwan and Andrea Rocker and Daniel Westphal and Max F. Hantke and Daniel P DePonte and Anton Barty and Joachim Schulz and Lars Gumprecht and Nicola Coppola and Andrew Aquila and Mengning Liang and Thomas A. White and Andrew Martin and Carl Caleman and Stephan Stern and Chantal Abergel and Virginie Seltzer and Jean-Michel Claverie and Christoph Bostedt and John D. Bozek and S{\'e}bastien Boutet and A. A. Miahnahri and Marc Messerschmidt and Jacek Krzywiński and Garth J. Williams and Keith O. Hodgson and Michael. Bogan and Christina Y. Hampton and Raymond G. Sierra and Dmitri Starodub and Inger Andersson and Sa{\vs}a Bajt and Miriam Barthelmess and John C. H. Spence and Petra Fromme and Uwe Weierstall and Richard A. Kirian and Mark S. Hunter and R. Bruce Doak and Stefano Marchesini and Stefan Hau-Riege and Matthias Frank and Robert L. Shoeman and Lukas Lomb and Sascha W Epp and Robert Hartmann and Daniel Rolles and Artem Rudenko and Carlo Schmidt and Lutz Foucar and Nils Kimmel and Peter Holl and Benedikt Rudek and Benjamin Erk and Andr{\'e} H{\"o}mke and Christian Reich and Daniel Pietschner and Georg Weidenspointner and Lothar Str{\"u}der and G{\"u}nter Hauser and Hubert Gorke and Joachim Ullrich and Ilme Schlichting and Sven C. Herrmann and Gerhard Schaller and Florian Schopper and Heike Soltau and K. U. K{\"u}hnel and Robert Andritschke and Claus Dieter Schr{\"o}ter and Faton Krasniqi and Mario Bott and Sebastian Schorb and Daniela Rupp and Marcus Adolph and Tais Gorkhover and Helmut Hirsemann and Guillaume Potdevin and Heinz Graafsma and Bj{\"o}rn Nilsson and Henry N. Chapman and Janos Hajdu},
X-ray lasers offer new capabilities in understanding the structure of biological systems, complex materials and matter under extreme conditions. Very short and extremely bright, coherent X-ray pulses can be used to outrun key damage processes and obtain a single diffraction pattern from a large macromolecule, a virus or a cell before the sample explodes and turns into plasma. The continuous diffraction pattern of non-crystalline objects permits oversampling and direct phase retrieval. Here we… 

Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

A dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source offers insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.

Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser

The dataset used for the first 3D reconstruction of a biological object from an X-ray FEL, which was the giant Mimivirus, is presented and could boost algorithm development and provide a benchmark dataset for new algorithms.

Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source

A dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source, which reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime.

Observation of a single protein by ultrafast X-ray diffraction

The idea of using ultrashort X-ray pulses to obtain images of single proteins frozen in time has fascinated and inspired many. It was one of the arguments for building X-ray free-electron lasers.

Optical and aerodynamic focusing of isolated particles for diffractive imaging experiments at X-ray free electron lasers

The highly brilliant, short and coherent pulses produced by X-ray free-electron lasers (XFELs) opened up a unique opportunity to image small objects in nature, such as protein macromolecules,

Data Descriptor: Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source

Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the

Virus Structures by X-Ray Free-Electron Lasers.

Until recently X-ray crystallography has been the standard technique for virus structure determinations, but the availability of XFELs led to the development of the method of serial femtosecond crystallography, where a crystal structure is determined from the measurement of hundreds to thousands of microcrystals.

X-ray lasers for structural and dynamic biology.

The use of femtosecond exposure times, rather than freezing of samples, as a means of minimizing radiation damage is shown to open up new opportunities for the molecular imaging of biochemical reactions at room temperature in solution.



Femtosecond diffractive imaging with a soft-X-ray free-electron laser

Theory predicts1,2,3,4 that, with an ultrashort and extremely bright coherent X-ray pulse, a single diffraction pattern may be recorded from a large macromolecule, a virus or a cell before the sample

Femtosecond diffractive imaging of biological cells

In a flash diffraction experiment, a short and extremely intense x-ray pulse illuminates the sample to obtain a diffraction pattern before the onset of significant radiation damage. The over-sampled

Potential for biomolecular imaging with femtosecond X-ray pulses

Computer simulations are used to investigate the structural information that can be recovered from the scattering of intense femtosecond X-ray pulses by single protein molecules and small assemblies and predict that ultrashort, high-intensity X-rays from free-electron lasers that are currently under development will provide a new approach to structural determinations with X- rays.

Femtosecond X-ray protein nanocrystallography

This work offers a new approach to structure determination of macromolecules that do not yield crystals of sufficient size for studies using conventional radiation sources or are particularly sensitive to radiation damage, by using pulses briefer than the timescale of most damage processes.

Femtosecond electronic response of atoms to ultra-intense X-rays

The nature of the electronic response in a free atom to unprecedented high-intensity, short-wavelength, high-fluence radiation is revealed and successful modelling of X-ray/atom interactions using a straightforward rate equation approach augurs favourably for extension to complex systems.

Quantitative imaging of single, unstained viruses with coherent x rays.

This work opens the door for quantitative x-ray imaging of a broad range of specimens from protein machineries and viruses to cellular organelles, and represents an experimental milestone towards the x-Ray imaging of large protein complexes.

Single particle X-ray diffractive imaging.

By intercepting electrospray-generated particles with a single 15 femtosecond soft-X-ray pulse, diffractive imaging of a nanoscale specimen in free flight for the first time is demonstrated, an important step toward imaging uncrystallized biomolecules.

Hawk: the image reconstruction package for coherent X‐ray diffractive imaging

The past few years have seen a tremendous growth in the field of coherent X-ray diffractive imaging, in large part due to X-ray free-electron lasers which provide a peak brilliance billions of times

Structure from Fleeting Illumination of Faint Spinning Objects in Flight with Application to Single Molecules

This work demonstrates the recovery of the structure of a weakly scattering macromolecule at the anticipated next-generation X-ray source intensities, and closes a critical gap in determining theructure of single molecules and nanoparticles byX-ray methods, and opens the way to reconstructing the structures of spinning, or randomly-oriented objects at extremely low signal levels.