Single copy shRNA configuration for ubiquitous gene knockdown in mice


RNA interference through the expression of small hairpin RNA (shRNA) molecules has become a very promising tool in reverse mouse genetics as it may allow inexpensive and rapid gene function analysis in vivo. However, the prerequisites for ubiquitous and reproducible shRNA expression are not well defined. Here we show that a single copy shRNA-transgene can mediate body-wide gene silencing in mice when inserted in a defined locus of the genome. The most commonly used promoters for shRNA expression, H1 and U6, showed a comparably broad activity in this configuration. Taken together, the results define a novel approach for efficient interference with expression of defined genes in vivo. Moreover, we provide a rapid strategy for the production of gene knockdown mice combining recombinase mediated cassette exchange and tetraploid blastocyst complementation approaches.

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@article{Seibler2005SingleCS, title={Single copy shRNA configuration for ubiquitous gene knockdown in mice}, author={Jost Seibler and Birgit K{\"{u}ter-Luks and Heidrun Kern and Sandra Streu and Leona Plum and Jan Mauer and Ralf K{\"{u}hn and Jens C. Br{\"{u}ning and Frieder Schwenk}, journal={Nucleic Acids Research}, year={2005}, volume={33}, pages={e67 - e67} }