Single-copy primary transformants of maize obtained through the co-introduction of a recombinase-expressing construct

@article{Srivastava2001SinglecopyPT,
  title={Single-copy primary transformants of maize obtained through the co-introduction of a recombinase-expressing construct},
  author={Vibha Srivastava and David Ow},
  journal={Plant Molecular Biology},
  year={2001},
  volume={46},
  pages={561-566}
}
We describe a variation of the method to generate single-copy transgenic plants by recombinase-mediated resolution of multiple insertions. In this study, a transgene construct flanked by oppositely oriented lox sites was co-bombarded into maize cells along with a cre-expressing construct. From analysis of the regenerated plants, a high percentage of the primary transformants harbored a single copy of the introduced transgene, and among these, a majority also lacked the cre construct. We deduce… CONTINUE READING