Single-channel recording of ligand-gated ion channels

  title={Single-channel recording of ligand-gated ion channels},
  author={Martin Mortensen and Trevor G Smart},
  journal={Nature Protocols},
Electrophysiological recording of single-channel currents is the most direct method available for obtaining detailed and precise information about the kinetic behavior of ion channels. A wide variety of cell types can be used for single-channel recording, but to obtain the highest resolution of the briefest channel opening and closing events, low-noise recordings, coupled with a minimal filtering frequency, are required. Here, we present a protocol designed to help those with some… CONTINUE READING
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