Single-chain immunotoxin fusions between anti-Tac and Pseudomonas exotoxin: relative importance of the two toxin disulfide bonds.

@article{Kreitman1993SinglechainIF,
  title={Single-chain immunotoxin fusions between anti-Tac and Pseudomonas exotoxin: relative importance of the two toxin disulfide bonds.},
  author={Robert J. Kreitman and Janendra K. Batra and Shakuntla Seetharam and Vijay K Chaudhary and David J P Fitzgerald and Ira Pastan},
  journal={Bioconjugate chemistry},
  year={1993},
  volume={4 2},
  pages={112-20}
}
Anti-Tac(Fv)-PE40 is a recombinant single-chain immunotoxin in which the variable heavy and light domains of the anti-IL2 receptor antibody, anti-Tac, are connected to each other by a peptide linker and then fused to PE40, a truncated form of Pseudomonas exotoxin (PE). This fusion protein has four disulfide bonds: one in each of the two variables domains, one in domain II (Cys 265-287), and one in domain Ib (Cys 372-379) of PE. To study the importance of the disulfide bonds of the toxin to the… CONTINUE READING

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