Single chain des-(B30) insulin. Intramolecular crosslinking of insulin by trypsin catalyzed transpeptidation.

@article{Markussen1985SingleCD,
  title={Single chain des-(B30) insulin. Intramolecular crosslinking of insulin by trypsin catalyzed transpeptidation.},
  author={J. B. Markussen and Knud Jorgensen and Anders Robert Sorensen and Lars Thim},
  journal={International journal of peptide and protein research},
  year={1985},
  volume={26 1},
  pages={
          70-7
        }
}
Single chain des-(B30) insulin (SCI) has been synthesized from porcine insulin by trypsin in a medium with a low content of water. Trypsin catalyzes an intramolecular transpeptidation reaction in which the glycineA1 residue substitutes the alanineB30 residue, rendering a LysB29 -GlyA1 peptide link between the A- and B-chains of insulin. The insulin derivative has been purified by column chromatography and appears to be homogeneous in HPLC and disc electrophoresis. The structure was proven to be… CONTINUE READING

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