Single-chain antibody-based immunotoxins targeting Her2/neu: design optimization and impact of affinity on antitumor efficacy and off-target toxicity.

@article{Cao2012SinglechainAI,
  title={Single-chain antibody-based immunotoxins targeting Her2/neu: design optimization and impact of affinity on antitumor efficacy and off-target toxicity.},
  author={Yu juan Cao and James D Marks and Qian Huang and Stephen I. Rudnick and Chiyi Xiong and Walter N. Hittelman and Xiaoxia Wen and John W. Marks and Lawrence H. Cheung and Kim Boland and Chun Sheng Li and Gregory P. Adams and Michael G. Rosenblum},
  journal={Molecular cancer therapeutics},
  year={2012},
  volume={11 1},
  pages={143-53}
}
Recombinant immunotoxins, consisting of single-chain variable fragments (scFv) genetically fused to polypeptide toxins, represent potentially effective candidates for cancer therapeutics. We evaluated the affinity of various anti-Her2/neu scFv fused to recombinant gelonin (rGel) and its effect on antitumor efficacy and off-target toxicity. A series of rGel-based immunotoxins were created from the human anti-Her2/neu scFv C6.5 and various affinity mutants (designated ML3-9, MH3-B1, and B1D3… CONTINUE READING
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