Single cell systems biology by super-resolution imaging and combinatorial labeling

@inproceedings{Lubeck2012SingleCS,
  title={Single cell systems biology by super-resolution imaging and combinatorial labeling},
  author={Eric Lubeck and Long Cai},
  booktitle={Nature Methods},
  year={2012}
}
Fluorescence microscopy is a powerful quantitative tool for exploring regulatory networks in single cells. However, the number of molecular species that can be measured simultaneously is limited by the spectral overlap between fluorophores. Here we demonstrate a simple but general strategy to drastically increase the capacity for multiplex detection of molecules in single cells by using optical super-resolution microscopy (SRM) and combinatorial labeling. As a proof of principle, we labeled… CONTINUE READING
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