Single-cell sequencing

  title={Single-cell sequencing},
  author={Tal Nawy},
  journal={Nature Methods},
  • Tal Nawy
  • Published 2013
  • Medicine, Biology
  • Nature Methods
  • Genome and transcriptome sequencing require orders of magnitude more starting material than what is found in an individual cell, pushing the limits of amplification technology. Handling such small quantities means that degradation, sample loss and contamination can have a pronounced effect on sequence quality and robustness. Heavy amplification also propagates errors and biases, which can lead to uneven coverage, noise and inaccurate quantification. Recent technical advances have helped… CONTINUE READING
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