Single-cell multiplexed cytokine profiling of CD19 CAR-T cells reveals a diverse landscape of polyfunctional antigen-specific response

@inproceedings{Xue2017SinglecellMC,
  title={Single-cell multiplexed cytokine profiling of CD19 CAR-T cells reveals a diverse landscape of polyfunctional antigen-specific response},
  author={Qiong Xue and Emily Bettini and Patrick Paczkowski and Colin Leong Liong Ng and Alaina Kaiser and Timothy McConnell and Olja Kodrasi and M{\'a}ire F. Quigley and James R. Heath and Rong Fan and Sean D Mackay and Mark Edward Dudley and Sadik H. Kassim and Jing Zhou},
  booktitle={Journal for immunotherapy of cancer},
  year={2017}
}
BACKGROUND It remains challenging to characterize the functional attributes of chimeric antigen receptor (CAR)-engineered T cell product targeting CD19 related to potency and immunotoxicity ex vivo, despite promising in vivo efficacy in patients with B cell malignancies. METHODS We employed a single-cell, 16-plex cytokine microfluidics device and new analysis techniques to evaluate the functional profile of CD19 CAR-T cells upon antigen-specific stimulation. CAR-T cells were manufactured from… CONTINUE READING