Single-cell methods

  title={Single-cell methods},
  author={Natalie de Souza},
  journal={Nature Methods},
Gene biomarker prediction in glioma by integrating scRNA-seq data and gene regulatory network
  • Guimin Qin, Longting Du, Yuying Ma, Yu Yin, Liming Wang
  • Medicine
    BMC medical genomics
  • 2021
Background Although great efforts have been made to study the occurrence and development of glioma, the molecular mechanisms of glioma are still unclear. Single-cell sequencing technology provides a
Dynamic screening and printing of single cells using a microfluidic chip with dual microvalves.
An innovative microfluidic chip integrating two pneumatic microvalves to screen and print single cells onto a well plate enables dynamic regulation of both the lower and the upper size limits of cells for printing.
Gene Biomarker Prediction in Glioma by Integrating scRNA-seq Data and the Gene Regulatory Network
An algorithm framework to explore the molecular mechanisms of glioma by integrating single-cell gene expression profiles and gene regulatory relations showed that these candidate tumor marker genes were closely related toglioma and could provide clues for the diagnosis and prognosis of patients with gliomas.
High-throughput 3D imaging of single cells with light-sheet fluorescence microscopy on chip.
An optofluidic microscope-on-chip with integrated microlenses for light-sheet shaping and with a fluidic microchannel that allows the automatic and continuous delivery of samples of a few tens of microns in size is presented.
Progress and applications of single-cell sequencing techniques.
  • Aimaiti Yasen, Abudusalamu Aini, +7 authors H. Wen
  • Medicine, Biology
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
  • 2020
The methods and application fields of SCS are summarized, which may provide potential references for its future clinical applications, including the analysis of embryonic and organ development, the immune system, cancer progression, and parasitic and infectious diseases as well as stem cell research, antibody screening, and therapeutic research and development.
3C and 3C-based techniques: the powerful tools for spatial genome organization deciphering
The methodologies of C-technologies used in genome organization studies with an emphasis on Hi-C method are described and compared and it is considered that constantly improving technologies will offer a finer and more accurate contact profiles of entire genome and ultimately reveal specific molecular machines govern its shape and function.
Disposable Optical Stretcher Fabricated by Microinjection Moulding
The prototype presented in this work demonstrates the feasibility of the microinjection moulding combined with removable inserts approach, which should guarantee real mass production of ready-to-use lab-on-chip devices.
Exploring the interaction capacities of microorganisms by the use of high-resolution experimental tools
Cells of Saccharomyces cerevisiae poses a possible detoxification method for the removal of mycotoxins through the formation of a yeast-mycotoxin complex. One of the main fungal genus that produces
Stable Isotope Techniques for the Assessment of Host and Microbiota Response During Gastrointestinal Dysfunction.
The purpose of this review is to assess the state-of-the-art use of stable isotope technologies and to provide a perspective on where these technologies can be exploited to further the understanding of gut dysfunction in EED.
Determinants Enteric infections Social and environmental factors Nutrient intake Gut microbiome Gut function Genetic and epigenetic factors Outcomes Growth Congnitive development Vaccine
What Is Known Linear growth r 165 million chi Nutritional effects on linea environmental major contribut interventions.