Single-cell methods

@article{Souza2009SinglecellM,
  title={Single-cell methods},
  author={Natalie de Souza},
  journal={Nature Methods},
  year={2009},
  volume={7},
  pages={35-35}
}
The ability to study single cells will permit a better understanding of cellular heterogeneity. 
18 Citations
Lab-on-a-chip for optical manipulation of single cells
  • R. Osellame
  • Materials Science
    2013 Conference on Lasers and Electro-Optics Pacific Rim (CLEOPR)
  • 2013
We present a new class of integrated optical devices, fabricated by femtosecond laser micromachining, that allows for mechanical probing, fluorescence detection and sorting of single cells by means
Imaging-assisted hydrogel formation for single cell isolation
TLDR
The method presented here offers a mask-free, cost-efficient and easy-to-use alternative to many currently existing surface-based cell-sorting techniques, and has the potential to impact the field of cell culturing and isolation, e.g. single cell genomics and proteomics.
Progress and applications of single-cell sequencing techniques.
  • Aimaiti Yasen, Abudusalamu Aini, +7 authors H. Wen
  • Medicine, Biology
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
  • 2020
TLDR
The methods and application fields of SCS are summarized, which may provide potential references for its future clinical applications, including the analysis of embryonic and organ development, the immune system, cancer progression, and parasitic and infectious diseases as well as stem cell research, antibody screening, and therapeutic research and development.
Single-cell electroporation using a multifunctional pipette.
TLDR
A method to fabricate low-resistance metallic electrodes into a PDMS pipette is demonstrated, followed by characterization of its effectiveness, benefits and limits in comparison with an external carbon microelectrode.
Large-scale arrays of picolitre chambers for single-cell analysis of large cell populations.
TLDR
This new method consists of an array of microchambers in which individual cells are collected, enclosed, and lysed to create a reaction mixture of the cytoplasm with extracellular detection agents and the analysis compared well with conventional flow-cytometric evaluation of mixed cell populations.
Monitoring impedance changes associated with motility and mitosis of a single cell.
TLDR
The data demonstrate subtle electrical changes associated with cell motility and for the first time with division at the single-cell level and it is speculated that this could open up new avenues for characterizing healthy and pathological cells.
Lightning Fast and Highly Sensitive Full-Length Single-cell sequencing using FLASH-Seq
TLDR
FLASH-Seq (FS), a new scRNA-seq method which displays greater sensitivity while decreasing incubation times and reducing the number of processing steps compared to its predecessors, is developed.
Validation and perspectives of a femtosecond laser fabricated monolithic optical stretcher
TLDR
The production and use of a monolithic glass chip for optical stretching of white blood cells, featuring microfluidic channels and optical waveguides directly written into bulk glass by femtosecond laser pulses is demonstrated.
Analysis of gene expression levels in individual bacterial cells without image segmentation.
TLDR
An alternative method for analyzing studies of stochasticity in gene expression without segmentation is demonstrated, which employs a comparison between the pixel brightness in phase contrast vs fluorescence microscopy images to obtain well-defined estimates for the different levels of gene expression that are present in the cell or cluster.
Cytocompatible Hydrogel Composed of Phospholipid Polymers for Regulation of Cell Functions
We propose a cell encapsulation matrix for use with a cytocompatible phospholipid polymer hydrogel system for control of cell functions in three-dimensional (3D) cell engineering and for construction
...
1
2
...

References

SHOWING 1-2 OF 2 REFERENCES
Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum
TLDR
Single-cell “mass cytometry” analyses provide system-wide views of immune signaling in healthy human hematopoiesis, against which drug action and disease can be compared for mechanistic studies and pharmacologic intervention.
Single-cell dissection of transcriptional heterogeneity in human colon tumors
TLDR
It is demonstrated that the transcriptional diversity of cancer tissues is largely explained by in vivo multilineage differentiation and not only by clonal genetic heterogeneity.