Single-cell measurement of superoxide anion and hydrogen peroxide production by human neutrophils with digital imaging fluorescence microscopy.

@article{Szcs1998SinglecellMO,
  title={Single-cell measurement of superoxide anion and hydrogen peroxide production by human neutrophils with digital imaging fluorescence microscopy.},
  author={S{\'a}ndor Szűcs and Gy{\"o}rgy V{\'a}mosi and R{\'o}bert P{\'o}ka and Attila S{\'a}rv{\'a}ry and Helga B{\'a}rdos and Margit Bal{\'a}zs and J{\'a}nos Kappelmayer and L. Toth and J{\'a}nos Sz{\"o}llősi and R{\'o}za {\'A}d{\'a}ny},
  journal={Cytometry},
  year={1998},
  volume={33 1},
  pages={19-31}
}
Besides flow cytometry, fluorescence microscopy combined with computerized image analysis offers an alternative tool for assessing phagocyte oxidant generation at the single-cell level. This technique provides an opportunity for the direct visualization of cells and simultaneous measurement of cellular fluorescence intensity. Thus, we developed a simple method for the quantitative evaluation of intracellular superoxide anion and hydrogen peroxide production with image cytometry by using… CONTINUE READING

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