Single-cell in situ RNA profiling by sequential hybridization

During each round of hybridization, we targeted each transcript by a set of FISH probes labeled with a single type of fluorophore. We imaged the sample and then treated it with DNAse I to remove the FISH probes. In a subsequent round the mRNA was hybridized with the same FISH probes, but now labeled with a different dye. The number of barcodes available… (More)

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