Single-cell imaging of Wnt palmitoylation by the acyltransferase porcupine.

  title={Single-cell imaging of Wnt palmitoylation by the acyltransferase porcupine.},
  author={Xinxin Gao and Rami N. Hannoush},
  journal={Nature chemical biology},
  volume={10 1},
Wnts are secreted palmitoylated glycoproteins that are important in embryonic development and human cancers. Here we report a method for imaging the palmitoylated form of Wnt proteins with subcellular resolution using clickable bioorthogonal fatty acids and in situ proximity ligation. Palmitoylated Wnt3a is visualized throughout the secretory pathway and trafficks to multivesicular bodies that act as export sites in secretory cells. We establish that glycosylation is not required for Wnt3a… CONTINUE READING
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