Single-cell genetic analysis of ductal carcinoma in situ and invasive breast cancer reveals enormous tumor heterogeneity yet conserved genomic imbalances and gain of MYC during progression.

@article{HeselmeyerHaddad2012SinglecellGA,
  title={Single-cell genetic analysis of ductal carcinoma in situ and invasive breast cancer reveals enormous tumor heterogeneity yet conserved genomic imbalances and gain of MYC during progression.},
  author={Kerstin Heselmeyer-Haddad and Lissa Y Berroa Garcia and Amanda I. Bradley and Clarymar Ortiz-Melendez and Woei-Jyh Lee and Rebecca L Christensen and Sheila Prindiville and Kathleen A Calzone and Peter W. Soballe and Yue Hu and Salim Akhter Chowdhury and Russell Schwartz and Alejandro A. Sch{\"a}ffer and Thomas Ried},
  journal={The American journal of pathology},
  year={2012},
  volume={181 5},
  pages={1807-22}
}
Ductal carcinoma in situ (DCIS) is a precursor lesion of invasive ductal carcinoma (IDC) of the breast. To understand the dynamics of genomic alterations in this progression, we used four multicolor fluorescence in situ hybridization probe panels consisting of the oncogenes COX2, MYC, HER2, CCND1, and ZNF217 and the tumor suppressor genes DBC2, CDH1, and TP53 to visualize copy number changes in 13 cases of synchronous DCIS and IDC based on single-cell analyses. The DCIS had a lower degree of… CONTINUE READING