Single-cell gel electrophoresis (Comet) assay in nano-genotoxicology.


The Comet assay, or single-cell gel electrophoresis assay, is an easy and simple yet reliable method to evaluate DNA damage in cells. Under the alkaline conditions of this method, DNA strand breaks and alkaline-labile sites are detected. Here we describe the alkaline version of the Comet assay with applications in testing nanoparticles.

DOI: 10.1007/978-1-62703-002-1_4

Cite this paper

@article{Pacurari2012SinglecellGE, title={Single-cell gel electrophoresis (Comet) assay in nano-genotoxicology.}, author={Maricica Pacurari and Vincent Castranova}, journal={Methods in molecular biology}, year={2012}, volume={926}, pages={57-67} }