Single-cell enzyme concentrations, kinetics, and inhibition analysis using high-density hydrodynamic cell isolation arrays.

@article{Carlo2006SinglecellEC,
  title={Single-cell enzyme concentrations, kinetics, and inhibition analysis using high-density hydrodynamic cell isolation arrays.},
  author={Dino Di Carlo and Nima Aghdam and Luke P. Lee},
  journal={Analytical chemistry},
  year={2006},
  volume={78 14},
  pages={4925-30}
}
High-quality single-cell data are required for a quantitative systems biology description of cellular function. However, data of this type are difficult and time-consuming to collect using traditional techniques. We present a robust and simple microfluidic method for trapping single cells in large arrays to address this problem. Ordered single-cell isolation arrays allow for high-density microscopic analysis with simplified image processing. Moreover, for fluorescent assays, on-chip sample… CONTINUE READING
Highly Cited
This paper has 560 citations. REVIEW CITATIONS
88 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 88 extracted citations

561 Citations

050100'09'12'15'18
Citations per Year
Semantic Scholar estimates that this publication has 561 citations based on the available data.

See our FAQ for additional information.

Similar Papers

Loading similar papers…