Single-cell enzymatic dissociation of hESC lines OxF1-OxF4 and culture in feeder-free conditions.

@article{Brook2012SinglecellED,
  title={Single-cell enzymatic dissociation of hESC lines OxF1-OxF4 and culture in feeder-free conditions.},
  author={Frances Anne Brook},
  journal={Methods in molecular biology},
  year={2012},
  volume={873},
  pages={209-15}
}
Experimental manipulation of hESCs has been hampered by their fragility and susceptibility to apoptosis when dissociated into single cells. The OxF lines are particularly robust and may be successfully passaged as single cells, with the inclusion of ROCK inhibitor in the medium. The protocols here describe the enzymatic dissociation of hESCs into a single… CONTINUE READING