Single cell digital polymerase chain reaction on self-priming compartmentalization chip.


Single cell analysis provides a new framework for understanding biology and disease, however, an absolute quantification of single cell gene expression still faces many challenges. Microfluidic digital polymerase chain reaction (PCR) provides a unique method to absolutely quantify the single cell gene expression, but only limited devices are developed to… (More)
DOI: 10.1063/1.4975192