Single-cell barcoding and sequencing using droplet microfluidics

@article{Zilionis2017SinglecellBA,
  title={Single-cell barcoding and sequencing using droplet microfluidics},
  author={R. Zilionis and J. Nainys and A. Veres and Virginia Savova and D. Zemmour and Allon M. Klein and L. Mazutis},
  journal={Nature Protocols},
  year={2017},
  volume={12},
  pages={44-73}
}
  • R. Zilionis, J. Nainys, +4 authors L. Mazutis
  • Published 2017
  • Biology, Medicine
  • Nature Protocols
  • Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for capturing the unbiased diversity of cells. Droplet microfluidics is among the most promising candidates for capturing and processing thousands of individual cells for whole-transcriptome or genomic analysis in a massively parallel manner with minimal reagent use. We recently established a method called inDrops, which… CONTINUE READING
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