Single-cell barcoding and sequencing using droplet microfluidics

  title={Single-cell barcoding and sequencing using droplet microfluidics},
  author={Rapolas Zilionis and Juozas Nainys and Adrian Veres and Virginia Savova and David Zemmour and Allon M. Klein and Linas Mazutis},
  journal={Nature Protocols},
Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for capturing the unbiased diversity of cells. Droplet microfluidics is among the most promising candidates for capturing and processing thousands of individual cells for whole-transcriptome or genomic analysis in a massively parallel manner with minimal reagent use. We recently established a method called inDrops, which… 

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