Single-cell barcoding and sequencing using droplet microfluidics

  title={Single-cell barcoding and sequencing using droplet microfluidics},
  author={R. Zilionis and J. Nainys and A. Veres and Virginia Savova and D. Zemmour and Allon M. Klein and L. Mazutis},
  journal={Nature Protocols},
  • R. Zilionis, J. Nainys, +4 authors L. Mazutis
  • Published 2017
  • Biology, Medicine
  • Nature Protocols
  • Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for capturing the unbiased diversity of cells. Droplet microfluidics is among the most promising candidates for capturing and processing thousands of individual cells for whole-transcriptome or genomic analysis in a massively parallel manner with minimal reagent use. We recently established a method called inDrops, which… CONTINUE READING
    281 Citations

    Figures and Topics from this paper.

    Inertial-ordering-assisted droplet microfluidics for high-throughput single-cell RNA-sequencing.
    • 29
    • PDF
    Multiplexed droplet single-cell RNA-sequencing using natural genetic variation
    • 240
    • PDF
    Single‐Cell Analysis Using Droplet Microfluidics
    • 6
    Comparative Analysis of Droplet-Based Ultra-High-Throughput Single-Cell RNA-Seq Systems.
    • 66
    • PDF
    A diffusion-based microfluidic device for single-cell RNA-seq.
    • 3
    • PDF
    SMARTer single cell total RNA sequencing
    • 10
    • PDF


    High-throughput microfluidic single-cell RT-qPCR
    • 366
    • PDF
    Scalable microfluidics for single-cell RNA printing and sequencing
    • 88
    • PDF
    Microfluidic single-cell whole-transcriptome sequencing
    • 205
    • PDF