Single cell assay of exocytosis from pancreatic islet B cells

@article{Gillis1992SingleCA,
  title={Single cell assay of exocytosis from pancreatic islet B cells},
  author={Kevin D. Gillis and Stanley Misler},
  journal={Pfl{\"u}gers Archiv},
  year={1992},
  volume={420},
  pages={121-123}
}
We have measured changes in membrane capacitance (ΔCm) in pancreatic B cells as a single cell assay of insulin secretion. Evidence that depolarization evoked ΔCm reflects exocytosis includes it's voltage, temperature and Ca2+ o dependence. Decreases in Cm, presumably reflecting endocytosis, occur on a variable time scale. Two features that make B cells unique among excitable cells are the large magnitude of ΔCm, when normalized to Ca2+ current, and the rapid “fatigue” of the response for even… CONTINUE READING

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Direct measurement of exocytosis and calcium currents in single vertebrate nerve terminals

N. C. Lira, M. C. Nowycky, R. J. Bookman
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