Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor.

@article{Fiering1990SingleCA,
  title={Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor.},
  author={Steven Fiering and Jeffrey P. Northrop and Garry P. Nolan and Pertti Mattila and Gerald R Crabtree and Leonard A. Herzenberg},
  journal={Genes & development},
  year={1990},
  volume={4 10},
  pages={1823-34}
}
Stimulation of T lymphocytes through their antigen receptor leads to the appearance of several transcription factors, including NF-AT and NF-kappa B, which are involved in regulating genes required for immunologic activation. To investigate the activity of a single transcription factor in individual viable cells, we have applied an assay that uses the fluorescence-activated cell sorter to quantitate beta-galactosidase (beta-gal). We have analyzed the distribution of NF-AT transcriptional… CONTINUE READING