Single cell analysis applied to antibody fragment production with Bacillus megaterium: development of advanced physiology and bioprocess state estimation tools

@inproceedings{David2011SingleCA,
  title={Single cell analysis applied to antibody fragment production with Bacillus megaterium: development of advanced physiology and bioprocess state estimation tools},
  author={Florian David and Antje Berger and Robert Haensch and Manfred Rohde and Ezequiel Franco-Lara},
  booktitle={Microbial cell factories},
  year={2011}
}
BACKGROUND Single cell analysis for bioprocess monitoring is an important tool to gain deeper insights into particular cell behavior and population dynamics of production processes and can be very useful for discrimination of the real bottleneck between product biosynthesis and secretion, respectively. RESULTS Here different dyes for viability estimation considering membrane potential (DiOC2(3), DiBAC4(3), DiOC6(3)) and cell integrity (DiBAC4(3)/PI, Syto9/PI) were successfully evaluated for… CONTINUE READING

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