Single cell PCR from archival stained bone marrow slides: a method for molecular diagnosis and characterization.

@article{Zanssen2004SingleCP,
  title={Single cell PCR from archival stained bone marrow slides: a method for molecular diagnosis and characterization.},
  author={Stefanie Zanssen},
  journal={Journal of clinical laboratory analysis},
  year={2004},
  volume={18 3},
  pages={176-81}
}
Molecular analysis of isolated single cells is a powerful tool for clarifying issues of cell origin and clonality. Previous reports have described PCR amplifications from total DNA and RNA extracted from archival bone marrow and peripheral blood smears and have also shown the feasibility of amplifications from single cells, microdissected from stained histological sections. In this study, a method is described for performing PCR from morphologically defined single cells isolated from archival… CONTINUE READING
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