Single-base mutations at position 2661 of Escherichia coli 23S rRNA increase efficiency of translational proofreading.

@article{Melanon1992SinglebaseMA,
  title={Single-base mutations at position 2661 of Escherichia coli 23S rRNA increase efficiency of translational proofreading.},
  author={P. Melançon and William E Tapprich and L{\'e}a Brakier-Gingras},
  journal={Journal of bacteriology},
  year={1992},
  volume={174 24},
  pages={
          7896-901
        }
}
Two single-base substitutions were constructed in the 2660 loop of Escherichia coli 23S rRNA (G2661-->C or U) and were introduced into the rrnB operon cloned in plasmid pKK3535. Ribosomes were isolated from bacteria transformed with the mutated plasmids and assayed in vitro in a poly(U)-directed system for their response to the misreading effect of streptomycin, neomycin, and gentamicin, three aminoglycoside antibiotics known to impair the proofreading control of translational accuracy. Both… CONTINUE READING
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