Single antibody domains as small recognition units: design and in vitro antigen selection of camelized, human VH domains with improved protein stability.

@article{Davies1996SingleAD,
  title={Single antibody domains as small recognition units: design and in vitro antigen selection of camelized, human VH domains with improved protein stability.},
  author={J. Eric Davies and Lutz Riechmann},
  journal={Protein engineering},
  year={1996},
  volume={9 6},
  pages={
          531-7
        }
}
Folding stabilities of camelized human antibody VH domains were studied through the determination of their melting points in thermodenaturation experiments. The melting point of a VH domain originating from a synthetic library of human VHs, which had been optimized for the use as small recognition units through the mimicking of camelid antibody heavy chains occurring naturally without light chain, was 56.6 degrees C compared with 71.2 degrees C of the original human VH. Its stability was… CONTINUE READING

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Serrano.L., KelhsJ.T., Cann.P., MatouschekA and Fersht.A

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  • Riechmann.L. and Weill.M
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