Single- and double-color oligonucleotide primed in situ labeling (PRINS): applications in pathology

  title={Single- and double-color oligonucleotide primed in situ labeling (PRINS): applications in pathology},
  author={L. Wilkens and Jo{\"e}lle Tchinda and Paul Komminoth and Martin Werner},
  journal={Histochemistry and Cell Biology},
 Molecular cytogenetics is mostly performed by fluorescence in situ hybridization using long DNA probes that are generated by vector cloning. Oligonucleotide primed in situ labeling (PRINS) is a recent method that has been established for the detection of the centromeric or telomeric region in metaphase chromosomes. In this overview, we demonstrate the possible applications of PRINS and provide elaborated protocols for its use in intact interphase cells of routine cytological preparations, e.g… CONTINUE READING
6 Citations
40 References
Similar Papers


Publications referenced by this paper.
Showing 1-10 of 40 references

Applications of single and double color digonucleotide primed in situ labeling ( PRINS ) in cytology Mod Pathol

  • M Werner, A Nasarek, J Tchinda, R Wasielewski, P Komminoth, L Wilkens
  • 1997

Rapid detection of karyotype changes in interphase bone marrow cells by oligonucleotide primed in situ labeling ( PRINS )

  • L Wilkens, P Komminoth, A Nasarek, Wasielewski R von, M Werner
  • J Pathol
  • 1997

Rapid brightfield detection of oligonucleotide primed in situ ( PRINS ) labeled DNA in chromosome preparations and frozen tissue sections

  • Speel EJM, D Lawson, Ramaekers FCS, Gosden, Hopman AHN
  • Biotechniques
  • 1996

Similar Papers

Loading similar papers…