Single amino acid substitutions influencing the folding pathway of the phage P22 tail spike endorhamnosidase.

Temperature-sensitive mutations in the gene for the thermostable tail spike of phage P22 interyFere with the folding and subunit association pathway at the restrictive temperature but not with the activity or stability of the protein once matured. The local sites of these mutations and the mutant amino acid substitutions have been determined by DNA… CONTINUE READING