Single amino acid substitutions in V(H) CDR2 are sufficient to generate or enhance the specificity of two forms of an anti-arsonate antibody variable region for DNA.

Abstract

We previously showed that a variety of amino acid substitutions at positions 58 and 59 in the V(H) CDR2 of an anti-arsonate (Ars) antibody Fab simultaneously resulted in increased or unaltered affinity for Ars and substantially enhanced affinity for DNA. To test the generality of these observations, we generated and characterized several antibody phage… (More)

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