Single amino acid changes can influence titer, heparin binding, and tissue tropism in different adeno-associated virus serotypes.

@article{Wu2006SingleAA,
  title={Single amino acid changes can influence titer, heparin binding, and tissue tropism in different adeno-associated virus serotypes.},
  author={Zhijian Wu and Aravind Asokan and Joshua C. Grieger and Lakshmanan Govindasamy and Mavis Agbandje-McKenna and Richard Jude Samulski},
  journal={Journal of virology},
  year={2006},
  volume={80 22},
  pages={11393-7}
}
Despite the high degree of sequence homology between adeno-associated virus (AAV) serotype 1 and 6 capsids (99.2%), these viruses have different liver transduction profiles when tested as vectors. Examination of the six amino acid residues that differ between AAV1 and AAV6 revealed that a lysine-to-glutamate change (K531E) suppresses the heparin binding ability of AAV6. In addition, the same mutation in AAV6 reduces transgene expression to levels similar to those achieved with AAV1 in HepG2… CONTINUE READING