Single-Stranded Antisense siRNAs Guide Target RNA Cleavage in RNAi

@article{Martinez2002SingleStrandedAS,
  title={Single-Stranded Antisense siRNAs Guide Target RNA Cleavage in RNAi},
  author={Javier Ruiz Martinez and Agnieszka Patkaniowska and Henning Urlaub and Reinhard L{\"u}hrmann and Thomas Tuschl},
  journal={Cell},
  year={2002},
  volume={110},
  pages={563-574}
}
Small interfering RNAs (siRNAs) are the mediators of mRNA degradation in the process of RNA interference (RNAi). Here, we describe a human biochemical system that recapitulates siRNA-mediated target RNA degradation. By using affinity-tagged siRNAs, we demonstrate that a single-stranded siRNA resides in the RNA-induced silencing complex (RISC) together with eIF2C1 and/or eIF2C2 (human GERp95) Argonaute proteins. RISC is rapidly formed in HeLa cell cytoplasmic extract supplemented with 21 nt… CONTINUE READING
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RNAi as random

  • C. Lipardi, Q. Wei, B. M. Paterson
  • 2001
Highly Influential
4 Excerpts

beads were once again resuspended in buffer B followed by a second round of UV treatment for 15 minutes. Both eluates

  • E. Billy, V. Brondani, H. Zhang, U. Muller, W. Filipowicz
  • 2001
Highly Influential
5 Excerpts

ARGONAUTE1 is required

  • R.W, G. M. Rubin
  • RNaseD. Cell
  • 2002

Cleavage reactions were stopped by the addition

  • W. Deng, H. Lin
  • Proteinase K, dissolved in 50 mM Tris- Cell
  • 2002
1 Excerpt

Expanding small RNA interference

  • T.
  • Science
  • 2002
2 Excerpts

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