Single-Step Generation of Conditional Knockout Mouse Embryonic Stem Cells.

  title={Single-Step Generation of Conditional Knockout Mouse Embryonic Stem Cells.},
  author={Maty{\'a}{\vs} Flemr and Marc B{\"u}hler},
  journal={Cell reports},
  volume={12 4},
Induction of double-strand DNA breaks (DSBs) by engineered nucleases, such as CRISPR/Cas9 or transcription activator-like effector nucleases (TALENs), stimulates knockin of exogenous DNA fragments via homologous recombination (HR). However, the knockin efficiencies reported so far have not allowed more complex in vitro genome modifications such as, for instance, simultaneous integration of a DNA fragment at two distinct genomic sites. We developed a reporter system to enrich for cells with… CONTINUE READING
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