Single-Reaction, Multiplex, Real-Time RT-PCR for the Detection, Quantitation, and Serotyping of Dengue Viruses

@inproceedings{Waggoner2013SingleReactionMR,
  title={Single-Reaction, Multiplex, Real-Time RT-PCR for the Detection, Quantitation, and Serotyping of Dengue Viruses},
  author={Jesse J Waggoner and Janaki Abeynayake and Malaya K. Sahoo and Lionel Gresh and Yolanda T{\'e}llez and Karla Gonzalez and Gabriela Ballesteros and Anna Maria Pierro and Paolo Gaibani and Frances P. Guo and Vittorio Sambri and {\'A}ngel Balmaseda and K. H. M. C. T. Karunaratne and Ethan Harris and Benjamin A Pinsky},
  booktitle={PLoS neglected tropical diseases},
  year={2013}
}
BACKGROUND Dengue fever results from infection with one or more of four different serotypes of dengue virus (DENV). Despite the widespread nature of this infection, available molecular diagnostics have significant limitations. The aim of this study was to develop a multiplex, real-time, reverse transcriptase-PCR (rRT-PCR) for the detection, quantitation, and serotyping of dengue viruses in a single reaction. METHODOLOGY/PRINCIPAL FINDINGS An rRT-PCR assay targeting the 5' untranslated region… CONTINUE READING
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