Single Mutation in Shine-Dalgarno-Like Sequence Present in the Amino Terminal of Lactate Dehydrogenase of Plasmodium Effects the Production of an Eukaryotic Protein Expressed in a Prokaryotic System

@article{iek2012SingleMI,
  title={Single Mutation in Shine-Dalgarno-Like Sequence Present in the Amino Terminal of Lactate Dehydrogenase of Plasmodium Effects the Production of an Eukaryotic Protein Expressed in a Prokaryotic System},
  author={Mustafa Çiçek and Ozal Mutlu and Aysegul Demirhan Erdemir and Ebru Ozkan and Yunus Sariçay and Dilek Turgut-Balik},
  journal={Molecular Biotechnology},
  year={2012},
  volume={54},
  pages={602-608}
}
One of the most important step in structure-based drug design studies is obtaining the protein in active form after cloning the target gene. In one of our previous study, it was determined that an internal Shine-Dalgarno-like sequence present just before the third methionine at N-terminus of wild type lactate dehydrogenase enzyme of Plasmodium falciparum prevent the translation of full length protein. Inspection of the same region in P. vivax LDH, which was overproduced as an active enzyme… 
Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli
TLDR
Using computational and biochemical approaches, it is demonstrated that an internal translation initiation from a GTG codon within eNISTmAb results from a weak Shine–Dalgarno sequence and is facilitated by a putative ribosomal protein S1-binding site.

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