Single Multiplex PCR Assay To Identify Simultaneously the Six Categories of Diarrheagenic Escherichia coli Associated with Enteric Infections

@article{Vidal2005SingleMP,
  title={Single Multiplex PCR Assay To Identify Simultaneously the Six Categories of Diarrheagenic Escherichia coli Associated with Enteric Infections},
  author={M. Vidal and E. Kruger and C. Dur{\'a}n and Rosanna Lagos and M. Levine and V. Prado and C. Toro and R. Vidal},
  journal={Journal of Clinical Microbiology},
  year={2005},
  volume={43},
  pages={5362 - 5365}
}
ABSTRACT We designed a multiplex PCR for the detection of all categories of diarrheagenic Escherichia coli. This method proved to be specific and rapid in detecting virulence genes from Shiga toxin-producing (stx1, stx2, and eae), enteropathogenic (eae and bfp), enterotoxigenic (stII and lt), enteroinvasive (virF and ipaH), enteroaggregative (aafII), and diffuse adherent (daaE) Escherichia coli in stool samples. 
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References

SHOWING 1-10 OF 23 REFERENCES
Multiplex PCR for Diagnosis of Enteric Infections Associated with Diarrheagenic Escherichia coli
TLDR
A multiplex PCR for detection of three categories of diarrheagenic Escherichia coli was developed and enterohemorrhagic E. coli was identified in fecal samples from patients with hemorrhagic colitis, watery diarrhea, or hemolytic-uremic syndrome and from food-borne outbreaks. Expand
Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shiga-like toxin type by mismatch amplification mutation assay-multiplex PCR
Mismatch amplification mutation assay primers, specific for a unique base substitution in uidA of Escherichia coli O157:H7, was coupled with primers for the Shiga-like toxin I (SLT-I) and SLT-IIExpand
Multiplex PCR assay and simple preparation method for stool specimens detect enterotoxigenic Escherichia coli DNA during course of infection
TLDR
A nonradioactive multiplex PCR assay that provides a sensitive and specific method for detecting the presence of either or both toxin genes has been developed and results for samples from a human volunteer study of ETEC infection indicated that this method of sample preparation results in greater clinical sensitivity. Expand
A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells.
TLDR
The ability of enteropathogenic Escherichia coli to form attaching and effacing intestinal lesions is a major characteristic of EPEC pathogenesis and a chromosomal gene (eae) that is necessary for this activity is identified using TnphoA mutagenesis. Expand
[Temporal variation of genotypes and serotypes of enterohemorrhagic E Coli isolated from Chilean children with intestinal infections or hemolytic uremic syndrome].
TLDR
There was a temporal variation in toxigenic genotypes of enterohemorrhagic E. Coli strains, but no association between these genotypes and the risk for hemolytic uremic syndrome was observed. Expand
Enteroaggregative Escherichia coli.
TLDR
The pathogenesis of EAEC infection is not well understood, but a model can be proposed in which EAEC adhere to the intestinal mucosa and elaborate enterotoxins and cytotoxin, which result in secretory diarrhea and mucosal damage. Expand
Typical and Atypical Enteropathogenic Escherichia coli
Typical and atypical enteropathogenic Escherichia coli (EPEC) strains differ in several characteristics. Typical EPEC, a leading cause of infantile diarrhea in developing countries, is rare inExpand
Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent.
  • M. Levine
  • Biology, Medicine
  • The Journal of infectious diseases
  • 1987
There are four major categories of diarrheagenic Escherichia coli: enterotoxigenic (a major cause of travelers' diarrhea and infant diarrhea in less-developed countries), enteroinvasive (a cause ofExpand
ESCHERICHIA COLI STRAINS THAT CAUSE DIARRHŒA BUT DO NOT PRODUCE HEAT-LABILE OR HEAT-STABLE ENTEROTOXINS AND ARE NON-INVASIVE
TLDR
Three enteropathogenic Escherichia coli strains isolated from outbreaks of infantile diarrhoea and one strain from the "normal" colonic flora of a healthy adult and fed in doses of 10(6), 10(8), and 10(10) organisms in NaHCO3 to adult volunteers gave negative results in sensitive tests for heat-labile (L.T.T.) enterotoxin, invasiveness, and gross fluid accumulation. Expand
Virulence of enterohemorrhagic Escherichia coli: role of molecular crosstalk.
  • V. L. Tesh
  • Biology, Medicine
  • Trends in microbiology
  • 1998
TLDR
Many bacterial exotoxins, originally defined by cytopathic effects, may also possess additional biological activities that contribute to 'molecular crosstalk' between Shiga toxins and the cytokines made in response to these toxins during the development of disease. Expand
...
1
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3
...